Chrysalin (TP-508)

Description

What is Chrysalin (TP-508)?

Chrysalin (TP-508) is also referred to as rusalatide acetate. It is a synthetic peptide consisting of 23 amino acids that corresponds to residues 508–530 of human prothrombin, representing the high-affinity receptor-binding domain of the native thrombin molecule. Unlike proteolytically active thrombin, TP-508 does not activate the coagulation cleavage cascade. Instead, it is investigated in laboratory settings for its interactions with a distinct subset of thrombin receptors on fibroblasts, endothelial cells, and stem cells.

TP-508 is not a SARM, not a hormone analog, and not a dietary supplement. It is not approved by the FDA for human or veterinary use and is intended strictly for laboratory research purposes.

Chemical Properties

Property	Detail
Product Type	Peptide
Product Name	Chrysalin (TP-508)
Format	Lyophilized Vial
Application	Scientific / Research Use Only
CAS	121341-81-9
Molecular Weight	2,312.44 g/mol
Chemical Formula	C₉₇H₁₄₆N₂₈O₃₆S
PubChem CID	91668177
Peptide Length	23 amino acids
Sequence	Ala-Gly-Tyr-Lys-Pro-Asp-Glu-Gly-Lys-Arg-Gly-Asp-Ala-Cys-Glu-Gly-Asp-Ser-Gly-Gly-Pro-Phe-Val
Peptide Source	Residues 508–530 of human prothrombin
IUPAC Name	H-Ala-Gly-Tyr-Lys-Pro-Asp-Glu-Gly-Lys-Arg-Gly-Asp-Ala-Cys-Glu-Gly-Asp-Ser-Gly-Gly-Pro-Phe-Val-OH
Synonyms	TP508, Chrysalin®, rusalatide acetate, TRAP-508
Physical Form	White to off-white lyophilized powder
Solubility	Soluble in sterile water or 0.1% acetic acid solution
Storage	−20°C, protected from light and moisture
Classification	Research Use Only (RUO)

Vial format note: Chrysalin (TP-508) is supplied as a lyophilized powder in a sterile, sealed glass vial with a black flip-top aluminum crimp cap. This format maintains peptide integrity under recommended cold storage conditions. The acetate salt form is the standard supplied form for most research applications.

How Does Chrysalin (TP-508) Work?

Nonproteolytic Thrombin Receptor Binding and eNOS Activation

TP-508 has been investigated in preclinical cell culture systems as a nonproteolytic agonist at a distinct subset of thrombin receptors expressed on fibroblasts and endothelial cells. In isolated endothelial cell systems, TP-508 has been observed to stimulate endothelial nitric oxide synthase (eNOS) activation and rapid NO production.

Angiogenic signaling and Endothelial Cell Motility

In preclinical animal wound healing models, TP-508 has been observed to stimulate endothelial cell chemokinesis and chemotaxis in a dose-dependent manner. Studies show increased vascularization markers at wound sites in TP-508-treated groups compared to saline controls.

Fibroblast Gene Expression Pathway

In isolated fibroblast cell systems, TP-508 has been investigated for its potential to modulate differential gene expression through a nonproteolytic receptor activation pathway. Preclinical data suggest this may involve upstream signaling events distinct from those initiated by proteolytically active thrombin.

Stem Cell Proliferation Pathway

In in vitro model systems, TP-508 has been investigated for its potential to affect stem cell proliferation signaling. Preclinical data from adipose tissue-derived stem cell cultures suggest that TP-508 may interact with the PI3K/Akt pathway.

What are the Potential Research Applications of Chrysalin (TP-508)?

In controlled laboratory environments, Chrysalin (TP-508) vials have been investigated for the following research applications.

• Nonproteolytic thrombin receptor binding kinetics and structure-activity relationship (SAR) studies
• eNOS activation and nitric oxide signaling pathway research
• Angiogenesis assay reference compound in preclinical wound healing models
• αvβ3 integrin-mediated signal transduction research
• Fibroblast gene expression pathway investigation
• Comparative thrombin fragment pharmacology studies

What are the Potential Side Effects of Chrysalin (TP-508)?

Researchers in preclinical and in vitro settings have noted the following observations.

• No human safety data has been established for this research-grade formulation
• Long-term or chronic toxicity data do not exist for this research preparation
• Dose-dependent endothelial cell motility responses observed in preclinical wound healing models
• PI3 kinase/Akt pathway interactions observed in adipose-derived stem cell culture
• Data from preclinical models remains limited. Evidence is not consistent across all experimental conditions

Risk & Handling

Chrysalin (TP-508) is a synthetic, nonproteolytic thrombin peptide. It is under investigation in preclinical research.

Risk Tier: MODERATE

This rating reflects TP-508’s bioactive receptor signaling activity at thrombin receptors, the bioactive concentrations used in preclinical research, and the need for proper handling to prevent accidental skin or inhalation exposure during reconstitution. Researchers must observe the following precautions at all times.

Handling Precautions: Chrysalin (TP-508) vials should be handled exclusively in a controlled laboratory environment by trained personnel. Use appropriate PPE at all times. Avoid direct skin and eye contact during reconstitution. Avoid inhalation.

Exposure Risks: No human safety data has been established for the research-grade formulation supplied here. Long-term or chronic toxicity data do not exist.

Storage: Store lyophilized TP-508 vials at −20°C in a dry, dark environment. Protect from light, heat, and moisture at all times. Avoid repeated freeze-thaw cycles. The vial seal should remain intact until use.

FAQs

What makes TP-508 nonproteolytic in research contexts?

TP-508 does not activate the proteolytic cleavage cascade associated with thrombin’s coagulation function otherwise. In preclinical settings, it interacts with a distinct receptor subset. This makes it a useful tool for isolating receptor-mediated signaling events in experimental models.

Is Chrysalin (TP-508) safe for human use?

No. Chrysalin (TP-508) provided by RCD Bio is not approved by the FDA for human or veterinary use. Please review the Terms and Conditions before ordering.

What is the vial format for Chrysalin (TP-508) at RCDBio?

Chrysalin (TP-508) is supplied as a lyophilized powder in a sterile, sealed vial with a flip-top aluminum crimp cap. This format maintains peptide integrity under recommended cold storage conditions. It is the standard for research-grade lyophilized peptides.

References

1. Olszewska-Pazdrak, B., Hart-Vantassell, A., & Carney, D. H. (2010). Thrombin peptide TP508 stimulates rapid nitric oxide production in human endothelial cells. Journal of Vascular Research, 47(3), 203–213. https://pubmed.ncbi.nlm.nih.gov/19893317/
2. Hembrough, T. A., Swartz, G. M., Papathanassiu, A., et al. (2001). Thrombin peptide, TP508, stimulates angiogenic responses in animal models of dermal wound healing, in chick chorioallantoic membranes, and in cultured human aortic and microvascular endothelial cells. Journal of Vascular Research. https://pubmed.ncbi.nlm.nih.gov/11888680/
3. Sower, L. E., Payne, D. A., Meyers, R., & Carney, D. H. (1999). Thrombin peptide, TP508, induces differential gene expression in fibroblasts through a nonproteolytic activation pathway. Experimental Cell Research, 247(2), 422–431. https://pubmed.ncbi.nlm.nih.gov/10066370/
4. Freyberg, S., Song, Y. H., Muehlberg, F., & Alt, E. (2009). Thrombin peptide (TP508) promotes adipose tissue-derived stem cell proliferation via PI3 kinase/Akt pathway. Journal of Vascular Research, 46(2), 98–102. https://pubmed.ncbi.nlm.nih.gov/18607113/

Disclaimer

Chrysalin (TP-508) is exclusively for laboratory research purposes. RCDbio products are not intended to diagnose, prevent, treat, or cure any disease or medical condition.

The Food and Drug Administration has not evaluated the statements on our website. This product is not approved for human or veterinary use. Researchers must comply with all applicable local, state, and federal laws and regulations governing the purchase and use of research compounds. By purchasing, you agree to our Terms and Conditions. RCDbio reserves the right to refuse sales to unauthorized individuals.

ATTENTION: All RCDbio products are strictly for LABORATORY AND RESEARCH PURPOSES ONLY. They are not intended for human consumption, veterinary use, or any other non-research application. For queries, complaints, or support, contact support@rcdbio.co